HISTOCHEMICAL LOCALIZATION OF SPECIFIC OXIDATIVE ENZYMES I. TETRAZOLIUM STAINS FOR DIPHOSPHOPYRIDINE NUCLEOTIDE DIAPHORASE AND TRIPHOSPHOPYRIDINE NUCLEOTIDE DIAPHORASE
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چکیده
منابع مشابه
The Histochemical Localization of Triphosphopyridine Nucleotide Diaphorase
A histochemical method is described for the localization of triphosphopyridine nucleotide diaphorase using a recently synthesized tetrazolium salt (Nitro-BT). By virtue of the favorable histochemical properties of this reagent, it has been possible to demonstrate that whereas DPN diaphorase is usually restricted to the mitochondria, the TPN diaphorase activity of corresponding cells was distrib...
متن کاملA Histochemical Method for the Demonstration of Diphosphopyridine Nucleotide Diaphorase
The present investigation concerning the histochemical demonstration of DPN diaphorase follows the development of a new reagent, Nitro-BT, which has already been used successfully for the cytochemical localization of the succinic dehydrogenase system. The most consistently favorable results were obtained with the lactate-lactic dehydrogenase system buffered at pH 7.4. Using sections of rat kidn...
متن کاملTriphosphopyridine Nucleotide Diaphorase from Wheat Germ.
11. KREBS, H. A. and HENSELEIT, K. Untersuchungen iiber die harnstoffbildung im tierkorper. Zeit. physiol. Chem. 210: 33. 1932. 12. McELROY, W. D. and GLASS, H. B. Amino acid Metabolism. Pp. 300-320. Johns Hopkins Press, Baltimore 1955. 13. MUELLER, G. C., QUINN, E. M. and RUECKERT, R. R. The formation of 1-phosphoerythrulose-4-C" by homogenates of Swiss chard leaves. Arch. Biochem. Biophys. 55...
متن کاملPurification and properties of diphosphopyridine nucleotide diaphorase of human erythrocytes.
Diaphorase Assay-Six-hundredths pmole of 2,6-dichlorophenolindophenol, 50 pmoles of Tris-HCl, pH 7.55, 1 pmole of EDTA, enzyme, and 0.44 pmole of DPNH in a volume of 3 ml were placed in a Beckman spectrophotometer equipped with a water jacket through which water at 25” was circulated. A control cuvette without enzyme was included, and absorbancy at 600 rnp was determined for 10 minutes. Under t...
متن کاملLevels of oxidized and reduced diphosphopyridine nucleotide and triphosphopyridine nucleotide in tumours.
Estabrook, R. W. (1955). Fed. Proc. 14, 45. Goddard, D. R. (1944). Amer. J. Bot. 31, 270. Hill, R. (1954). Nature, Lond., 174, 501. Hill, R. & Bhagvat, K. (1939). Nature, Lond., 143, 726. Hill, R. & Hartree, E. F. (1953). Annu. Rev. Pl. Physiol. 4, 115. Hill, R. & Scarisbrick, R. (1951). New Phytol. 50, 98. Holton, F. A. (1955). Biochem. J. 61, 46. Keilin, D. (1925). Proc. Roy. Soc. B, 98, 312....
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1956
ISSN: 0022-1554,1551-5044
DOI: 10.1177/4.3.254